881 Establishment of in vitro model of IL-36Ra dysfunction in a human keratinocyte cell line by IL36RN knockout using CRISPR/Cas9 system

IL-36Ra, a member of the IL-1 family, is an antagonist IL-36 receptor and inhibitory regulator cascade. Loss function mutations in IL36RN gene, which encodes have been demonstrated to enhance cascade subsequently activation MAPK NF-κB pathways, resulting neutrophil recruitment pustule formation skin by increased production chemokines such as IL-8 CXCL-1. Cutaneous inflammation caused dysregulated expression however, has not yet led elucidation role keratinocytes or involvement other pro-inflammatory cytokines skin, might lead identification new therapeutic molecular targets. To investigate effects IL-36Ra human keratinocytes, we knocked out gene HaCaT cells using CRISPR/Cas9 genome editing system. Here describe applications methods editing.